KMID : 0880220110490060994
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Journal of Microbiology 2011 Volume.49 No. 6 p.994 ~ p.999
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Antibacterial efficacy of lytic Pseudomonas bacteriophage in normal and neutropenic mice models
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Birendra R. Tiwari
Kim Shuk-Ho Marzia Rahman Kim Jung-Min
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Abstract
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Recently, lytic bacteriophages (phages) have been focused on treating bacterial infectious diseases. We investigated the protective efficacy of a novel Pseudomonas aeruginosa phage, PA1¨ª, in normal and neutropenic mice. A lethal dose of P. aeruginosa PAO1 was administered via the intraperitoneal route and a single dose of PA1¨ª with different multiplicities of infection (MOI) was treated into infected mice. Immunocompetent mice infected with P. aeruginosa PAO1 were successfully protected by PA1¨ª of 1 MOI, 10 MOI or 100 MOI with 80% to 100% survival rate. No viable bacteria were found in organ samples after 48 h of the phage treatment. Phage clearing patterns were different in the presence or absence of host bacteria but PA1¨ª disappeared from all organs after 72 h except spleen in the presence of host bacteria. On the contrary, PA1¨ª treatment could not protect neutropenic mice infected with P. aeruginosa PAO1 even though could extend their lives for a short time. In in vitro phage-neutrophil bactericidal test, a stronger bactericidal effect was observed in phage-neutrophil co-treatment than in phage single treatment without neutrophils, suggesting phage-neutrophil co-work is essential for the efficient killing of bacteria in the mouse model. In conclusion, PA1¨ª can be possibly utilized in future phage therapy endeavors since it exhibited strong protective effects against virulent P. aeruginosa infection.
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KEYWORD
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bacteriophage therapy, Pseudomonas aeruginosa, neutropenic mouse
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